FastQCFastQC Report
Sat 25 Oct 2025
stdin

Summary

[OK]Basic Statistics

MeasureValue
Filenamestdin
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000000
Total Bases150 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTGAATGGGGAATCAGGGTTCGATTCCGG18410.18409999999999999Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTGAATGGGCGCGACCTCAGATCAGACGT15470.1547Clontech Universal Primer Mix Long (100% over 23bp)
CCCAAGCATTATGGTAGTGGAAAGATCGGAAGAGCACACGTCTGAACTCC11540.1154Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)